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laserscanning confocal microscope  (Nikon)


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    Structured Review

    Nikon laserscanning confocal microscope
    Laserscanning Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laserscanning+confocal+microscope/C2%2B/pm41923531-49-14-19
    Average 99 stars, based on 1 article reviews
    laserscanning confocal microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Developmental Profiling of Structural and Functional Maturation in Mouse Corpus Callosum.
    Article Snippet: As the largest white matter tract within the central nervous system (CNS) to connect two cerebral hemispheres, the corpus callosum axon bundle consists of a mixture of myelinated and unmyelinated axons and plays a crucial role in executing sensory, motor and cognitive functions within the CNS.. In this study, we comprehensively characterized progressive alterations in myelination and oligodendrocyte lineage cell densities during the postnatal myelin development and then correlated these structural dynamics to the maturation of axonal impulse conduction within the mouse corpus callosum.. In addition, we found that the extracellular spaces between callosal axons were significantly reduced during the first three postnatal weeks in mice, while micronscale diffusion of small molecule within this region remained largely unaffected and displayed isotropy.

    Inverted Epifluorescence:

    Article Title: Developmental Profiling of Structural and Functional Maturation in Mouse Corpus Callosum.
    Article Snippet: As the largest white matter tract within the central nervous system (CNS) to connect two cerebral hemispheres, the corpus callosum axon bundle consists of a mixture of myelinated and unmyelinated axons and plays a crucial role in executing sensory, motor and cognitive functions within the CNS.. In this study, we comprehensively characterized progressive alterations in myelination and oligodendrocyte lineage cell densities during the postnatal myelin development and then correlated these structural dynamics to the maturation of axonal impulse conduction within the mouse corpus callosum.. In addition, we found that the extracellular spaces between callosal axons were significantly reduced during the first three postnatal weeks in mice, while micronscale diffusion of small molecule within this region remained largely unaffected and displayed isotropy.

    Microscopy:

    Article Title: Developmental Profiling of Structural and Functional Maturation in Mouse Corpus Callosum.
    Article Snippet: As the largest white matter tract within the central nervous system (CNS) to connect two cerebral hemispheres, the corpus callosum axon bundle consists of a mixture of myelinated and unmyelinated axons and plays a crucial role in executing sensory, motor and cognitive functions within the CNS.. In this study, we comprehensively characterized progressive alterations in myelination and oligodendrocyte lineage cell densities during the postnatal myelin development and then correlated these structural dynamics to the maturation of axonal impulse conduction within the mouse corpus callosum.. In addition, we found that the extracellular spaces between callosal axons were significantly reduced during the first three postnatal weeks in mice, while micronscale diffusion of small molecule within this region remained largely unaffected and displayed isotropy.

    Article Title: Differential actin binding along the PEVK domain of skeletal muscle titin.
    Article Snippet: .. Myofibrils were visualized in a laserscanning confocal microscope (Bio-Rad MRC 1024 attached to a Nikon Eclipse inverted microscope) using a 40× phase objective lens (0.75 N.A., Nikon Plan Fluor). ..

    Article Title: The essential role of phospho‐T38 CPI‐17 in the maintenance of physiological blood pressure using genetically modified mice
    Article Snippet: PKC-potentiated phosphorylation-dependent inhibitory protein of protein phosphatase 1 (CPI-17), an endogenous myosin phosphatase inhibitory protein, is considered a key molecule for Ca sensitization of the contractile apparatus.. Here, we have used clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 to generate CPI-17-deficient [knockout (KO)] and threonine 38 (T38)-phosphoresistantmice [Threoninemutant intoAlanine (TA)], and then effects ofCPI-17 onvascular contractility in vitro and meanbloodpressure (MBP) invivowere investigated.. In isolated thoracic aorta, phorbol 12, 13-dibutyrate induced a sustained contraction of wild-type (WT) mice, whereas no contraction showed from TA or KO mice.

    Article Title: Mmp14-dependent remodeling of the pericellular-dermal collagen interface governs fibroblast survival.
    Article Snippet: Dermal fibroblasts deposit type I collagen, the dominant extracellular matrix molecule found in skin, during early postnatal development.. Coincident with this biosynthetic program, fibroblasts proteolytically remodel pericellular collagen fibrils by mobilizing the membrane-anchored matrix metalloproteinase, Mmp14.. Unexpectedly, dermal fibroblasts in Mmp14−/− mice commit to a large-scale apoptotic program that leaves skin tissues replete with dying cells.

    Article Title: Increased stiffness of extracellular matrix enhanced chemoresistance in 3D-bioprinted ovarian cancer model
    Article Snippet: .. After incubation, the 3D-bioprinted model was gently washed with PBS and observed using a laserscanning confocal microscope (C2/C2si; Nikon, Tokyo, Japan). .. Five random fields were captured for each sample, and the cells in the five samples were counted using the ImageJ software (Version 1.8.0).

    Inverted Microscopy:

    Article Title: Differential actin binding along the PEVK domain of skeletal muscle titin.
    Article Snippet: .. Myofibrils were visualized in a laserscanning confocal microscope (Bio-Rad MRC 1024 attached to a Nikon Eclipse inverted microscope) using a 40× phase objective lens (0.75 N.A., Nikon Plan Fluor). ..

    Article Title: Mmp14-dependent remodeling of the pericellular-dermal collagen interface governs fibroblast survival.
    Article Snippet: Dermal fibroblasts deposit type I collagen, the dominant extracellular matrix molecule found in skin, during early postnatal development.. Coincident with this biosynthetic program, fibroblasts proteolytically remodel pericellular collagen fibrils by mobilizing the membrane-anchored matrix metalloproteinase, Mmp14.. Unexpectedly, dermal fibroblasts in Mmp14−/− mice commit to a large-scale apoptotic program that leaves skin tissues replete with dying cells.

    Incubation:

    Article Title: The essential role of phospho‐T38 CPI‐17 in the maintenance of physiological blood pressure using genetically modified mice
    Article Snippet: PKC-potentiated phosphorylation-dependent inhibitory protein of protein phosphatase 1 (CPI-17), an endogenous myosin phosphatase inhibitory protein, is considered a key molecule for Ca sensitization of the contractile apparatus.. Here, we have used clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 to generate CPI-17-deficient [knockout (KO)] and threonine 38 (T38)-phosphoresistantmice [Threoninemutant intoAlanine (TA)], and then effects ofCPI-17 onvascular contractility in vitro and meanbloodpressure (MBP) invivowere investigated.. In isolated thoracic aorta, phorbol 12, 13-dibutyrate induced a sustained contraction of wild-type (WT) mice, whereas no contraction showed from TA or KO mice.

    Article Title: Increased stiffness of extracellular matrix enhanced chemoresistance in 3D-bioprinted ovarian cancer model
    Article Snippet: .. After incubation, the 3D-bioprinted model was gently washed with PBS and observed using a laserscanning confocal microscope (C2/C2si; Nikon, Tokyo, Japan). .. Five random fields were captured for each sample, and the cells in the five samples were counted using the ImageJ software (Version 1.8.0).

    In Vitro:

    Article Title: Mmp14-dependent remodeling of the pericellular-dermal collagen interface governs fibroblast survival.
    Article Snippet: Dermal fibroblasts deposit type I collagen, the dominant extracellular matrix molecule found in skin, during early postnatal development.. Coincident with this biosynthetic program, fibroblasts proteolytically remodel pericellular collagen fibrils by mobilizing the membrane-anchored matrix metalloproteinase, Mmp14.. Unexpectedly, dermal fibroblasts in Mmp14−/− mice commit to a large-scale apoptotic program that leaves skin tissues replete with dying cells.



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